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February 2008, Volume: 44 Number: 2
Features
From the Editor
BioSpotlight
Citations
Scientists: Profile of Jeffrey V. Ravetch, M.D., Ph.D.
About the Authors:
WebWatch
Tech News
Troubleshooting Forum
NIH Grant Watch
BioFeedback
Benchmarks
Combining allele-specific fluorescent probes and restriction assay in real-time PCR to achieve SNP scoring beyond allele ratios of 1:1000
Jonci N. Wolff and Neil J. Gemmell
Assays for the rapid and specific identification of North American Yersinia pestis and the common laboratory strain CO92
Amy J. Vogler, Elizabeth M. Driebe, Judy Lee, Raymond K. Auerbach, Christopher J. Allender, Miles Stanley, Kristy Kubota, Gary L. Andersen, Lyndsay Radnedge, Patricia L. Worsham, Paul Keim, and David M. Wagner
Short Technical Reports
In-frame deletion of Escherichia coli essential genes in complex regulon
Rubing Liang and Jianhua Liu
Highly efficient deletion method for the engineering of plasmid DNA with single-stranded oligonucleotides
Lin-Yu Lu, Michael S.Y. Huen, Andrew Chi-Pang Tai, De-pei Liu, Kathryn S.E. Cheah, and Jian-Dong Huang
Scanning conductance microscopy investigations on fixed human chromosomes
Casper Hyttel Clausen, Jacob Moresco Lange, Linda Boye Jensen, Pranjul Jaykumar Shah, Maria Ioannou Dimaki, and Winnie Edith Svendsen
A genome walking strategy for the identification of eukaryotic nucleotide sequences adjacent to known regions
Claudia Leoni, Raffaele Gallerani, and Luigi R. Ceci
Real-time viability assay based on 51Cr retention in adherent cells
Lina Vennström, Camilla Bysell, Hanna Björkelund, Hans Lundqvist, and Karl Andersson
Research Reports
An oligonucleotide microarray for multiplex real-time PCR identification of HIV-1, HBV, and HCV
Dmitry A. Khodakov, Natalia V. Zakharova, Dmitry A. Gryadunov, Felix P. Filatov, Alexander S. Zasedatelev, and Vladimir M. Mikhailovich
Mammalian cell-seeded hydrogel microarrays printed via dip-pin technology
Isaac S. Baird, Amy Y. Yau, and Brenda K. Mann
Rapid capture of DNA targets
Judy St. John and Thomas W. Quinn
Construction and characterization of a normalized yeast two-hybrid library derived from a human protein-coding clone collection
Jorja Degrado-Warren, Max Dufford, Jian Chen, Paul L. Bartel, Donna Shattuck, and Georges C. Frech
BioTechniques for Preclinical Development 1
Introduction
First Steps on the Path
Douglas McCormick
Review
Translational science: past, present, and future
Stephen H. Curry
Short Technical Reports
Increased efficiency for performing colony formation assays in 96-well plates: novel applications to combination therapies and high-throughput screening
David Katz, Emma Ito, Ken S. Lau, Joseph D. Mocanu, Carlo Bastianutto, Aaron D. Schimmer, and Fei-Fei Liu
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